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¡¡¡¡Flow cytometric analysis of anti-CD3E CAR expression on anti-CD3E CAR-transfected 293FT cells. Cells were stained with PE-conjugated anti-G4S linker Antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Non-transduced 293FT cells were used as a control (right).
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¡¡¡¡Binding activity of PE-conjugated anti-G4S linker Antibody to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated anti-G4S linker Antibody and detected by flow cytometry. PE signal was used to evaluate the non-specific binding activity to PBMCs.
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